Desulphation of dextran sulphate during kidney ultrafiltration.

Partial Purification of Cold Active Lipase from Pseudomonas Sp. Vitclp4 Isolated from Marine Samples of Tamilnadu Coast

An extracellular cold active lipase from Pseudomonas sp. VITCLP4 was concentrated by two methods, ammonium sulphate precipitation and ultrafiltration. Ammonium sulphate precipitation with 40% saturation was found to give good results with 125-fold partially purified lipase and yield of 63%. Further purification by DEAE-Sephadex ion exchange chromatography resulted in 4.6-fold purified enzyme wi...

Metabolism of sodium oestrone ( 35 S) sulphate in the guinea pig.

Intraperitoneal administration of sodium oestrone [(35)S]sulphate to male and female free-ranging guinea pigs is followed by excretion of most of the radioactivity mainly as inorganic [(35)S]sulphate in the urine within 72h. The remainder of the radioactivity in the urine was found in oestrone [(35)S]sulphate, two unidentified metabolites (A and B) and traces of oestradiol-17beta 3-[(35)S]sulph...

Enzymic desulphation of l-serine O[S]-sulphate: the intracellular localization of the enzyme.

Histopathological effects of acute copper sulphate exposure on kidney, liver and gill of common carp, Cyprinus carpio, fingerlings

In the present study histological effects of copper sulphate on, 180 common carp, Cyprinus carpio, fingerlings were studied. In this regard, fish of treatment groups were exposed to 2, 4, 6, 8 and 10 ppm of copper sulphate during 4 days. Behaviours and clinical signs of fish were recorded during the experiment, and samples were done from kidney, liver and gill of moribund and fresh dead fish, a...

Decorin endocytosis: structural features of heparin and heparan sulphate oligosaccharides interfering with receptor binding and endocytosis.

Receptor-mediated endocytosis of decorin depends on its core-protein-mediated interaction with a 51 kDa membrane protein, which, in addition to its core-protein-binding site, carries a binding site for glycosaminoglycan chains. Membrane-associated heparan sulphate as well as heparin are known to have an inhibitory effect on decorin endocytosis by cultured skin fibroblasts. In this study, struct...